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In BN PAGE, the Coomassie G-250 binds to proteins and confers a net negative charge while maintaining the proteins in their native state without any protein denaturation.

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The Invitrogen NativePAGE Bis-Tris Gel System is a precast polyacrylamide mini-gel system that provides sensitive, high-resolution analysis of native proteins and protein complexes for molecular mass estimations, and assessment of purity.

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invitrogen bn page manual transfer

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BN-20 New Owners Guide: This 54 page PDF is a step by step walkthrough of the features of the VersaStudio BN-20 covering creation of files and output of specialty inks.

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Few things about blue native page I couldn't find at all. First, why do we have separate cathode and anode buffers (in traditional SDS PAGE tris-glycine is the only buffer) in BN PAGE?.

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